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BACEBiotility — University of FloridaProfessional CertificationIndustry-Recognized CredentialMultiple Choice

BACE: Applied Mathematics Practice Questions & Answers

Badge: Applied Mathematics

Type: Practical | Weight: 11.5% of Exam

Tests the ability to perform essential laboratory calculations and interpret graphical data. Requires numeric entry and graph analysis.

Formulas & Concepts:

  • Dilutions: C1V1=C2V2C_1V_1 = C_2V_2C1V1=C2V2 calculations.
  • Solutions: Molarity (M) and Percent solutions (w/v, v/v).
  • Data Handling: Significant figures and Scientific Notation.
  • Conversions: μLmL\mu L \leftrightarrow mLμLmL and other metric conversions.
  • Analysis: Standard curve interpretation from linear plots.

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A technician records a volume of 0.05040 L. How many significant figures does this measurement contain?

  • 3

  • 4

  • 5

  • 6

View Answer & Explanation
Correct Answer: Option B -

4

Explanation:

Leading zeros (0.0...) are never significant. Captive zeros (between non-zero digits, like the 0 in 504) are significant. Trailing zeros after a decimal point (the final 0) are significant to indicate precision. Therefore, the digits '5', '0', '4', '0' are significant.

Which of the following correctly expresses the number 0.0000458 in scientific notation?

  • 4.58×1044.58 \times 10^{-4}4.58×104

  • 4.58×1054.58 \times 10^{-5}4.58×105

  • 45.8×10645.8 \times 10^{-6}45.8×106

  • 4.58×1054.58 \times 10^{5}4.58×105

View Answer & Explanation
Correct Answer: Option B -

4.58×1054.58 \times 10^{-5}4.58×105

Explanation:

Move the decimal point 5 places to the right to get a number between 1 and 10 (4.584.584.58). Since the original number was less than 1, the exponent is negative: 4.58×1054.58 \times 10^{-5}4.58×105.

A protocol requires adding 450 μ\muμL of buffer to a tube. How many milliliters (mL) is this?

  • 0.045 mL

  • 0.45 mL

  • 4.5 mL

  • 450,000 mL

View Answer & Explanation
Correct Answer: Option B -

0.45 mL

Explanation:

There are 1,000 microliters (μ\muμL) in 1 milliliter (mL). To convert μ\muμL to mL, divide by 1,000: 4501000=0.45 mL\frac{450}{1000} = 0.45 \text{ mL}1000450=0.45 mL

Which of the following is the correct definition of Molarity (M)?

  • Moles of solute per kilogram of solvent

  • Grams of solute per liter of solution

  • Moles of solute per liter of solution

  • Milliliters of solute per 100 mL of solution

View Answer & Explanation
Correct Answer: Option C -

Moles of solute per liter of solution

Explanation:

Molarity (M) is defined as the number of moles of solute dissolved in one liter of total solution (mol/Lmol/Lmol/L).

You are provided with a 50X stock solution of TAE buffer. You need to prepare 1000 mL of 1X TAE buffer. How much stock solution and water do you need?

  • 50 mL stock + 950 mL water

  • 20 mL stock + 980 mL water

  • 10 mL stock + 990 mL water

  • 100 mL stock + 900 mL water

View Answer & Explanation
Correct Answer: Option B -

20 mL stock + 980 mL water

Explanation:

Use C1V1=C2V2C_1V_1 = C_2V_2C1V1=C2V2: (50X)(V1)=(1X)(1000 mL)(50X)(V_1) = (1X)(1000 \text{ mL})(50X)(V1)=(1X)(1000 mL). Solve for V1V_1V1: V1=100050=20 mLV_1 = \frac{1000}{50} = 20 \text{ mL}V1=501000=20 mL. To find the volume of water, subtract the stock volume from the total volume: 1000 mL20 mL=980 mL1000 \text{ mL} - 20 \text{ mL} = 980 \text{ mL}1000 mL20 mL=980 mL.

How many grams of agarose are needed to prepare 50 mL of a 2.5% (w/v) agarose gel?

  • 0.5 g

  • 1.25 g

  • 2.5 g

  • 12.5 g

View Answer & Explanation
Correct Answer: Option B -

1.25 g

Explanation:

Percent (w/v) means grams per 100 mL. A 2.5% solution means 2.5 g in 100 mL. For 50 mL (which is half of 100 mL), you need half the mass: 2.5 g×50 mL100 mL=1.25 g2.5 \text{ g} \times \frac{50 \text{ mL}}{100 \text{ mL}} = 1.25 \text{ g}2.5 g×100 mL50 mL=1.25 g.

A standard curve for protein concentration (xx​x-axis in mg/mL) vs. absorbance (yy​y-axis) yields the equation y=0.5x+0.02y = 0.5x + 0.02y=0.5x+0.02. If an unknown sample has an absorbance of 0.52, what is its concentration?

  • 0.25 mg/mL

  • 1.0 mg/mL

  • 1.08 mg/mL

  • 2.5 mg/mL

View Answer & Explanation
Correct Answer: Option B -

1.0 mg/mL

Explanation:

Substitute y=0.52y = 0.52y=0.52 into the equation and solve for xx​x: 0.52=0.5x+0.020.52 = 0.5x + 0.020.52=0.5x+0.020.50=0.5x0.50 = 0.5x0.50=0.5xx=0.500.5=1.0 mg/mLx = \frac{0.50}{0.5} = 1.0 \text{ mg/mL}x=0.50.50=1.0 mg/mL

Which micropipette would be most accurate for measuring 150 μ\muμL?

  • P20 (2–20 μ\muμL)

  • P200 (20–200 μ\muμL)

  • P1000 (100–1000 μ\muμL)

  • P10 (0.5–10 μ\muμL)

View Answer & Explanation
Correct Answer: Option B -

P200 (20–200 μ\muμL)

Explanation:

The P200 pipettor has a range of 20–200 μ\muμL. 150 μ\muμL falls squarely within this range. A P1000 could measure it, but P200 offers higher precision for this specific volume. P20 and P10 are too small.

The molecular weight of NaCl is 58.44 g/mol. How much NaCl is required to make 500 mL of a 0.5 M solution?

  • 14.61 g

  • 29.22 g

  • 58.44 g

  • 116.88 g

View Answer & Explanation
Correct Answer: Option A -

14.61 g

Explanation:

First calculate moles needed for 0.5 L (500 mL) at 0.5 M: Moles=M×L=0.5 mol/L×0.5 L=0.25 moles\text{Moles} = M \times L = 0.5 \text{ mol/L} \times 0.5 \text{ L} = 0.25 \text{ moles}Moles=M×L=0.5 mol/L×0.5 L=0.25 moles Then convert moles to grams: Mass=Moles×MW=0.25 mol×58.44 g/mol=14.61 g\text{Mass} = \text{Moles} \times \text{MW} = 0.25 \text{ mol} \times 58.44 \text{ g/mol} = 14.61 \text{ g}Mass=Moles×MW=0.25 mol×58.44 g/mol=14.61 g

Perform the following calculation and report the answer with the correct number of significant figures: 4.5×1.0024.5 \times 1.0024.5×1.002

  • 4.5

  • 4.50

  • 4.509

  • 4.51

View Answer & Explanation
Correct Answer: Option A -

4.5

Explanation:

In multiplication, the result must be rounded to the same number of significant figures as the factor with the fewest significant figures. 4.54.54.5 has 2 sig figs. 1.0021.0021.002 has 4 sig figs. The answer must be rounded to 2 sig figs. 4.5×1.002=4.5094.5 \times 1.002 = 4.5094.5×1.002=4.509, which rounds to 4.5.

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