BACE: Biotechnology Skills (Practical) Practice Questions & Answers

To ensure all equipment is cleaned with soap and water before use.

To prevent contamination of the culture, the environment, and the researcher.

To sterilize all solutions using high heat and pressure immediately after use.

To increase the growth rate of the bacterial culture being studied.

100°C at 0 psi for 60 minutes

121°C at 15 psi for 15-20 minutes

150°C at 30 psi for 5 minutes

200°C at 10 psi for 45 minutes

At the very front edge of the hood, closest to the user.

Outside the hood, transferring items in only when necessary.

At least 6 inches inside the hood, in the center of the sterile airflow.

Against the back wall of the hood to avoid air turbulence.

Flaming the mouth of a glass tube before and after transferring a sample.

Placing a sterile cap face-down on the lab bench while pipetting.

Holding the test tube at an angle while the cap is removed.

Using a fresh, sterile pipette tip for each transfer.

Flame loop $\rightarrow$→ Remove cap $\rightarrow$→ Flame tube lip $\rightarrow$→ Obtain sample $\rightarrow$→ Flame tube lip $\rightarrow$→ Recap

Remove cap $\rightarrow$→ Flame loop $\rightarrow$→ Obtain sample $\rightarrow$→ Flame tube lip $\rightarrow$→ Recap

Flame tube lip $\rightarrow$→ Flame loop $\rightarrow$→ Remove cap $\rightarrow$→ Obtain sample $\rightarrow$→ Recap

Obtain sample $\rightarrow$→ Flame loop $\rightarrow$→ Flame tube lip $\rightarrow$→ Remove cap $\rightarrow$→ Recap

To heat the liquid culture to boiling point.

To create an updraft that pushes airborne contaminants away from the open tube.

To illuminate the workspace.

To burn off excess oxygen in the tube.

Safety goggles

Gloves

Closed-toe shoes

Fire extinguisher

The broth has evaporated.

The broth is contaminated with microbial growth.

The pH of the broth has spontaneously changed.

The broth was over-sterilized.

Pass it quickly through the yellow part of the flame once.

Hold it in the blue cone of the flame until it glows red hot along the entire wire.

Hold it in the flame for exactly 3 seconds, regardless of color.

Dip it in ethanol and then waive it near the flame to dry.

The hot loop will melt the agar and ruin the plate.

The hot loop will kill the bacteria you are trying to pick up.

The hot loop will cause the bacteria to mutate.

The hot loop will cause aerosols that contaminate the air.